产品货号：
GS2084
中文名称：
PctI内切酶
英文名称：
PctI
产品规格：
250U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：PctI内切酶，PctI限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GAATGCN↑ |
| 来源 | Planococcus citreus SM |
| Assayed on | Lambda DNA |
| Optimal buffer | O?(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);250mMNaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100 μg/ml BSA;and 50%glycerol.Store at -20℃. |
| Ligations | After 20-fold overdigestion with enzyme 90%of the DNA fragments can be ligated and recut |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃ |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BsaMI,BscCI,BsmI,Mva1269I,Nb.BsmI^ |
相关搜索：PctI内切酶，PctI限制性内切酶